Table 1

Kinetic analysis of PP-1 mutants

PP-1Specific activity, units/mgKm, μMkcat, s−1kcat/Km, s−1·M−1 × 10−6Vmax, μmol·mg−1·min−1
Wild-type3410.6393.6363
N124D2.019.83.10.165.0
H248N0.75.10.50.100.8
D95A0.035.80.020.0030.03
R96A0.086.90.090.0130.2
R221S0.05105.00.20.0020.6
D208A8.023.313.30.5722
C127S4910.4585.5694
Y272F265.0142.8223
D220V308.1303.7049.3
E256R36NDNDNDND
E275R6913.2866.50140
E252A:D253A37NDNDNDND
E252A:D253A:E256R12NDNDNDND
  • ND, not determined.