Table 1

Rate of apoptosis of BAF/3-IL-2Rβ·ΔS cells stably transfected with CMV-6 or CMV-6-based expression constructs of catalytically active Akt mutants

CellsApoptosis, % dead cells
48 h72 h96 h
BAF/3·ΔS CMV-674  ±  7.596  ±  2.9100
BAF/3·ΔS·E40K-Akt52  ±  3.566  ±  3.375  ±  3.2
BAF/3·ΔS·Myr-Akt45  ±  4.152  ±  4.467  ±  4.5
  • Six independent cultures of BAF/3-IL-2Rβ·ΔS cells stably transfected with CMV-6 or CMV-6-based expression constructs of Akt E40K or myristylated Akt were cultured in the absence of IL-2 and IL-3 at the concentration of 0.5 × 106 cells per ml. Live and dead cells were counted daily and the percentage of dead cells (mean ± SD) at each time point was calculated as in Fig. 3A1. Surviving Akt E40K and myristylated Akt-expressing cells from all the cultures gave rise to growth factor-independent cell lines. Visible evidence of factor independent growth was observed within a week after growth factor deprivation.