Table 1

Requirement of LFA-1 affinity cycling for T cell locomotion

Cell treatment% adhesionAverage velocity,* μm/min% stopped
TS2/441  ±  4.611.6  ±  2.56.7 ± 2.5
KIM18592  ±  6.17.7  ±  1.343 ± 3.8
TS2/4 + mAb 2435  ±  3.75.1  ±  0.8343 ± 8.1
mAb 24 + KIM18594  ±  8.1100
  • * Average velocity is for moving cells only. ICAM-1 density is 500 molecules per μm2; KIM 185 and mAb 24 at 2 μg/ml; TS2/4 at 10 μg/ml. Stimulation of adhesion by KIM185 is a common observation (34). Data are presented as mean and range of two experiments in which 200 cells were measured for each value.