Table 2

Binding of [3H]muscimol and [3H]Ro15-4513 to postnatal day 1 mouse brain sections as revealed by autoradiography

Brain region[3H]Muscimol, nCi/mg[3H]Ro15-4513, nCi/mg
Frontoparietal cortex
 β3+/+0.80  ±  0.143.70  ±  0.84
 β3+/−0.72  ±  0.102.58  ±  0.33
 β3−/−0.58  ±  0.09*2.59  ±  0.52*
Olfactory bulbs
 β3+/+0.65  ±  0.082.43  ±  0.40
 β3+/−0.55  ±  0.072.16  ±  0.46
 β3−/−0.59  ±  0.051.00  ±  0.08
Caudate/putamen
 β3+/+0.66  ±  0.073.42  ±  0.63
 β3+/−0.64  ±  0.143.15  ±  0.34
 β3−/−0.53  ±  0.102.57  ±  0.37*
Thalamus
 β3+/+0.83  ±  0.164.92  ±  0.62
 β3+/−0.81  ±  0.223.79  ±  0.40
 β3−/−0.52  ±  0.06*2.51  ±  0.85
Hippocampus
 β3+/+0.70  ±  0.113.68  ±  0.90
 β3+/−0.64  ±  0.142.96  ±  0.80
 β3−/−0.57  ±  0.162.22  ±  0.48*
  • Optical density values in relation to radioactivity standards are means ± SEM for three to six brains. THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol) (10 μM) was able to reduce [3H]muscimol binding to the background level (data not shown). The statistical significance of differences between β3+/+, β3+/−, and β3−/− mice was determined using one-way ANOVA followed by Bonferroni post test (∗, P < 0.05;  

  • , P < 0.001).