Table 1

Paxillin binding to BPV-1 E6 mutants

E6 mutantFoci, n*Growth in agar*Paxillin binding
In vitroIn vivo
Wild-type68+100+
I41T64+81+
C128S26+93ND
C17P00ND
C20S00ND
C53R00ND
C90S00ND
C93H00ND
C93S00ND
H105D012
R116S019
C124V00ND
Δ127-13700ND
Δ134-137018
  • ND, not determined. 

  • * Data are derived from Vousden et al. (26). 

  • In vitro binding was determined by binding of in vitro-translated E6 products to GST–paxillin. 

  • In vivo binding was determined by coimmunoprecipitation of paxillin and FLAG-tagged E6 in COS cells.