Table 1

Analysis of complementation in DHP1 strain

PlasmidsPhenotype on MacConkey/maltoseβ-Galactosidase, units/mg dry weight bacteriacAMP, pmol/mg dry weight bacteriaAdenylate cyclase activity*
+CaM−CaM
NoneWhite179<10<1<0.01
pCm-AHL1Red/24 hr6,6503,40013,00010
pT25 + pT18White/72 hr130<10<1<0.01
pT25 + pT18-zipWhite/72 hr183<10<1<0.01
pT25-zip + pT18White/72 hr178<10<1<0.01
pT25-zip + pT18-zipRed/30 hr4,7501,10010,0004
  • Bacteria were grown in LB at 30°C in the presence of 0.5 mM isopropyl β-d-thiogalactoside plus appropriate antibiotics. The results represent the average values obtained for at least five independent cultures, which differed by less than 10%. 

  • * nmol cAMP/min per mg protein; when present in the assays, CaM was at a concentration of 1 μM.