Table 2

Complementation between various chimeric proteins

PlasmidsPhenotype on MacConkey/maltoseβ-Galactosidase, units/mg dry weight bacteriacAMP, pmol/mg dry weight bacteria
pT25-Tyr + pT18-TyrRed/40 hr2,800580
pT25-Tyr + pT18White/96 hr193<10
pT25 + pT18-TyrWhite/96 hr183<10
pT25-Tyr + pT18-zipWhite/96 hr134<10
pT25-zip + pT18-TyrWhite/96 hr126<10
pT25-prp11 + pT18-prp21Red/40 hr85065
  • Bacteria were grown in LB at 30°C in the presence of 0.5 mM isopropyl β-d-thiogalactoside plus appropriate antibiotics. The results represent the average values obtained for at least five independent cultures.