Table 1

Peptides from ESAT-6

HLA class I allelePeptide motifPeptideSequencePosition
HLA-A2-L/I/M------V/L/IES8GIEAAASAI10–18
ES9AIQGNVTSI17–25
ES10LLDEGKQSL28–36
ES11ELNNALQNL64–72
ES13 AMASTEGNV 82–90
HLA-B8--K-K---L/IES7EGKQSLTKL31–39
HLA-B52-Q-----I/VES12 LQNLARTI 69–76
  • We searched for 8-, 9-, or 10-residue sequences from ESAT-6, which were congruent with the peptide motifs of the HLA class I alleles -A2, -B7, -B8, -B35, -B52, and -B53. Sequences congruent with the peptide motifs for HLA-A2, HLA-B8, and HLA-B52 were identified; these peptides were synthesized and are displayed. No sequences congruent with HLA-B7, HLA-B35, and HLA-B53 were present in ESAT-6 and, thus, no peptides were synthesized for these HLA class I alleles. Peptides were sorted into pools that were used for in vitro restimulation of donor PBMCs. Peptides found to be CD8+ epitopes are shown in boldface. Similarly, 42 peptides were synthesized based on the sequences of antigens 85A and 85C. No CD8+ epitopes were identified among these and the peptides are not shown.