Table 1

Unbinding force values (Fu), dissociation constants (Kd), and Gibbs free energies of binding (ΔG) of wild-type and mutant His(H58)Ala

Cys-scFv-fragmentFu, pNKd, nMΔG, kJ/mol
Wild type50  ±  40.75−50.8
Mutant His(H58)Ala40  ±  38.94−44.8
  • Fu values with standard deviations are calculated from the means of five histograms.