Table 2

Proliferative response of the LNC

Source of LNC3H-thymidine incorporation, cpm ± SEM
NonemBSAConA
 ImmunizedIL-6 (+/+)622  ±  2846,526  ±  2,33045,908  ±  1,638
IL-6 (−/−)607  ±  2513,904  ±  305*44,827  ±  1,359
 Nonimmunized IL-6 (+/+)394  ±  29496  ±  129,264  ±  324
IL-6 (−/−)377  ±  18415  ±  1912,386  ±  1,435
  • Fourteen days after immunization with mBSA, mice were sacrificed. The inguinal lymph nodes were removed, and single-cell suspensions were prepared. The LNC (5 × 105 cells/well) were cultured with 0.2 ml of complete culture medium in the absence or presence of either mBSA (100 μg/ml) or ConA (1 μg/ml) in 96-well plates for 72 hr. Proliferative response was measured by 3H-thymidine incorporation. ∗, P < 0.001, IL-6 −/− compared to IL-6 +/+.