Table 1

Two-hybrid analysis of p70 and neurabin

Binding domain fusion protein (pPC97)Activation domain fusion protein (pPC86)Interaction assessed by β-gal filter assayInteraction assessed by histidine prototrophy
p70 (amino acids 332–502)neurabin (amino acids 486–751)++
neurabin (amino acids 486–751)p70 (amino acids 332–502)++
p70 (amino acids 332–502)cyclophilin
cyclophilinneurabin (amino acids 486–751)
p70 (amino acids 332–502)neurabin-GLAA (amino acids 486–751)
p70 Δ5 (amino acids 332–497)neurabin (amino acids 486–751)