Table 1

Thioredoxin reductase from HeLa cells

Retention time, minTotal protein, mg75Se, cpm/mgSp. activity, μmol/mg/min
DTNBTrx + glutathione disulfide*
Cells grown under 50% of saturation dissolved O2
 131.033.18  ×  10650.814.6
 430.271.78  ×  10614.76.4
Cells grown under 30% of saturation dissolved O2
 130.911.0  ×  10645.513.6
 430
  • Isoforms are separated by heparin affinity chromatography. 

  • * Similar activity is observed with oxidized insulin as disulfide substrate.