Table 1

Primers used for the amplification of variable (BV) and constant (BC) regions of the β chain of the murine TCR

ΔGTm, °CPositionSize, bp
BV1 5′-CGGTGCCCAGTCGTTTTATAC-3′ −40.853.8−89223
BV2 5′-CACACGGGTCACTGATACGGAG-3′ −41.056.7−61195
BV3 5′-CACCTTGCAGCCTAGAAATTCAG-3′ −42.554.9−50184
BV4 5′-CTGCCTCAAGTCGCTTCCAAC-3′ −41.256.3−89223
BV5 5′-TGARATGAACATGAGTGCCTTGG-3′ −41.356.4−43177
BV6 5′-TTCTCTCACTGTGACATCTGCCC-3′ −40.156.5−42176
BV7 5′-AAGGATACAGGGTCTCACGGAAG-3′ −42.755.3−80214
BV8 5′-GATRTCCCTGATGGRTACAAGGC-3′ −43.958.3−40174
BV8A 5′-TTGGCTTCCCTCTCTCAGACA-3′ −39.253.8−27161
BV9 5′-TCTCTCTACATTGGCTCTGCAGG-3′ −44.758.7−42176
BV10 5′-ATCAAGTCTGTAGAGCCGGAGG-3′ −41.154.1−33167
BV11 5′-AGGCACAAGGTGACAGGGAAG-3′ −40.455.5−243377
BV12 5′-ATGGTGGGGCTTTCAAGGATC-3′ −42.356.6−98232
BV13 5′-CTGTGAGGCCTAAAGGAACTAAC-3′ −40.751.1−68202
BV14 5′-ACGACCAATTCATCCTAAGCAC-3′ −40.152.5−53187
BV15 5′-CCATCAGTCATCCCAACTTATCC-3′ −41.454.0−69203
BV16 5′-GTGACCCTCAATTGTGACCCAG-3′ −40.455.6−213347
BV17 5′-CACGATACCTGGTCAAAGAGAAAG-3′ −42.253.9−245379
BV18 5′-CGGCCAAACCTAACATTCTC-3′ −41.955.1−63215
BC 5′-GGGTAGCCTTTTGTTTGTTTGC-3′ −42.554.684
BC-5′ 5′-GCTACCTTCTGGCACAATCCTC-3′ −41.254.5236
BC-3′ 5′-TAGGCATTTTCCAGGTCACAAG-3′ −40.653.5699463
GC clamp: 5′-CGCCCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCC-3′
  • Position +1 is defined as the first nucleotide 5′ to the sequence coding for the conserved amino acid sequence CASS in the proximal end of the variable region. The approximate size is calculated using an estimated length of the DJ region of 50 bp.