Table 2

Effect of soluble glycoproteins on interaction of buccal cells and E. coli strains KB91 (M1L) and KB96 (M1H)

Inhibitor*IC50
E. coli KB91 (M1L FimH)E. coli KB96 (M1H FimH)
Yeast mannan5.0  ± 0.30.5  ± 0.2
Bovine intestinal mucin1.7  ± 0.10.6  ± 0.1
Bovine RNase B0.9  ± 0.20.08  ± 0.02
Bovine lactoferrin2.1  ± 0.20.08  ± 0.03
  • * The ability of these E. coli strains to bind to the same glycoproteins immobilized on plastic was determined previously (21). The binding of KB91 and KB96 to the glycoproteins were, respectively, 0.5 ± 0.1 and 6.2 ± 0.2 to mannan, 0.4 ± 0.1 and 5.2 ± 0.2 to mucin, 7.5 ± 0.3 and 7.7 ± 0.2 to RNase B, and 6.1 ± 0.1 and 6.0 ± 0.1 to lactoferrin (mean cfu per well × 106, ± standard error of mean). 

  • The numbers given are the concentrations (mean mg per ml, ± standard error of mean) of each glycoprotein required to give 50% inhibition (i.e., IC50).