Table 1

Bacterial strains and plasmids used in this work

DesignationRelevant characteristics and useReference or source
Escherichia coli
 DH5αSupE44 ΔlacU169 (f80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1, NxrLife Technologies
 XLOLRΔ(mcrA) 183 Δ(mcrBC-hsdSMR-mrr)173 endA1 thi-1 recA1 gyrA96 relA1 lac [F′ proAB laqlqZΔM15 Tn10(Tcr)]Stratagene
 SM10λpirSM10 lysogenized with λ-pir for mobilizing pUT∷mini-Tn5Cm, Kmr 41
 MC4100F′ araD139 Δ(argF-lacZYA)U169 rpsL150 relA1 flb-5301 ptsF25 deoC1 42
Plasmids
 pFLAG-CTCFor construction of C-terminal fusion to FLAG peptide, AprKodak
 pUT∷mini- Tn5CmMini-Tn5 transposon with Cmr on suicide plasmid pGP704 derivative for transposon mutagenesis, Apr 43
 pML123Broad host range expression vector for cloning avrPto-FLAG, Gmr 44
 pPtE6An avrPto clone in pDSK519, Kmr 28
 pHIR11pLAFR3 carrying Pss61 hrp/hrc cluster, Tcr 45
 pCPP2156pCPP19 carrying E. chrysanthemi hrp cluster, SprThis work
 pCPP2157pCPP19 carrying E. chrysanthemi hrp cluster, Spr 19
 pCPP2329pFLAG-CTC carrying avrPto, AprThis work
 pAVRB-FLAG2pML123 carrying avrB-FLAG, Gmr 5
 pAVRB1pDSK519 carrying avrB, Kmr 27
 pAVRPTO-FLAGpML123 carrying avrPto-FLAG, GmrThis work
 pCPP2368pCPP2156∷Tn5Cm that has HR phenotype, Spr, CmrThis work
 pCPP2416pCPP2156∷Tn5Cm that has HR+ phenotype, Spr, CmrThis work
 pCPP2318pCPP30 carrying blaM lacking signal peptide sequences, Tcr 25
  • Ap, ampicillin; GM, gentamycin; Km, kanamycin; Nx, nalidixic acid.