Table 1

Phenotypes of different RPP loci in combination with wild-type or mutant eds1 or ndr1 after inoculation of selected F3 families with P. parasitica

RPP locus, P. parasitica isolateCrossF3 families, nGenotype*Phenotype
RPP2 (Cala2)Col-gl × Ler-eds1-25 RPP2, EDS1 R
5 RPP2, eds1-2 S
Col-ndr1-1 RPP2, ndr1-1 R
RPP4 (Emwa1)Col-gl × Ws-eds1-11 RPP4, EDS1 R
2 RPP4, eds1-1 S
Col-ndr1-1 RPP4, ndr1-1 R
RPP5 (Noco2)Col-ndr1-1 × Ler5 RPP5, NDR1 R
5 RPP5, ndr1-1 R
2 rpp5, NDR1 S
RPP8 (Emco5)Col-ndr1-1 × Ler5 RPP8, NDR1 R
5 RPP8, ndr1-1 R
Ws-0 rpp8, NDR1 S
  • * Each F3 family was homozygous for the respective RPP locus and wild-type or mutant allele. 

  • Twenty to 30 9-day-old seedlings were spray-inoculated with P. parasitica conidiospores. Resistance (R, no sporulation) or susceptibility (S, medium to profuse sporulation) was scored with a hand lens on the first true leaves 7 days after inoculation. 

  • Selfed Col ndr1, wild-type Colgl, or Ws-0 seedlings.