Table 2

HLA-DR3-restricted T cell responses and HLA-DR3 binding of 85B-derived peptides in humans or HLA-DR3.Ab0 mice

85B amino acid sequenceRecognition by HLA-DR3-restricted T cells*HLA-DR3 binding affinity, IC50 μM
In humansIn tg mice
 34-53  LQVPSPSMGRDIKVQFQSGG+++++0.4
154-173 SAMILAAYHPQQFIYAGSLS3
164-183 QQFIYAGSLSALLDPSQGMG8
134-153 ANRAVKPTGSAAIGLSMAGS9
144-163 AAIGLSMAGSSAMILAAYHP15
284-303 THSWEYWGAQLNAMKGDLQS25
All other peptides>100
  • DR3.Ab0 mice were immunized with BCG and Ag85 (10 μg per mouse). After 7 days, LNCs were challenged in vitro with 85B− peptide (50 μg/ml). 

  • * +++ indicates SI ≥ 4; ++ indicates 3 ≤ SI ≤ 4; − indicates SI < 2. 

  • Peptide binding affinity (IC50) was defined as high-affinity (<1 μM), intermediate-affinity (1 μM to 10 μM), weak-affinity (10 μM to 100 μM), or nonbinding (>100 μM), according to ref. 27