Table 1

Choline growth phenotype and enzyme activities in bet mutants

Strain (genotype)Growth*Enzyme activity
CDHBADHMDH
102F34R (wild type)100113 ± 2284 ± 17214 ± 8
UNA130 (betA2∷Ω)NGND61 ± 12214 ± 3
UNA156 (betB2∷Ω)NGNDND213 ± 2
UNA208 (betC4∷Tn5)NGNDND185 ± 31
UNA210 (betI5∷Tn5)20 18 ± 2 3 ± 1 186 ± 20
UNA137 (betCBAΔΩ)NGNDND190 ± 7
  • NG, no growth improvement compared with LAS + 0.5 M NaCl (<10% wild-type growth). ND, not detected (CDH, <10 nmol/min per mg; BADH, <1 nmol/min per mg). 

  • * The ability of the strains to use choline as osmoprotectant was determined in LAS + 0.5 M NaCl + 1 mM choline. Values represent the percentage of wild-type growth. 

  • Cells were grown in MCAA + choline. Enzyme activity was determined from triplicate assays and given in nmol/min per mg of protein ± SEM.