Table 2

TCR Vα and Vβ families used by β cell-cytotoxic CD8+ T cell lines

MouseT Cells
1AI482
2AI11.A51716
AI11.A717n.d.
AI11.B22, 17, 188, 13
3AI12.B1∗4, 10, 178, 11, 16
4AI15.A31, 3, 4, 5, 13, 17, 206, 8, 10, 11, 13, 14
AI15.A1058
5AI15.F5178
  • CD8+ T cells were isolated from the islets of prediabetic 5- to 6-week-old NOD mice, and TCR Vα and Vβ family usage was determined by reverse transcription–PCR. PCR products found to represent nonproductive gene rearrangements are not included. Vα and Vβ gene families were assigned according to Arden et al.(23). n.d., not determined. 

  • * This line was later sorted into three clonotypes: Vα10/Vβ16, Vα4/Vβ11, and Vα17/Vβ8.