Table 4

TCR β chain CDR3 sequences used by β cell-autoreactive CD8+ T cells

MouseCellsFWCDR3FW
1AI42CTCSAHRGLGNTLYFG1.3
2AI11.A516CASSSDWGYEQYFG2.6
AI11.B28S1CASSSDTYEQYFG2.6
13CASSRTGGRGEQYFG2.6
3AI12.B1.116CASSLARPSAETLYFG2.3
AI12.B1.211CASSLGDWGNEQYFG2.6
AI12.B1.38S3CASSGDRYEQYFG2.6
4AI15.A36CASSRSGTGTEVFFG1.1
8S1CASSGTGGQNTLYFG2.4
10CASSLGQYEQYFG2.6
11CASSWTGGGTLYFG2.4
13CASIKLGGQNTLYFG2.4
14CAWSHSYYNSPLYFA1.6
AI15.A108S2CASGDAGGDQAPLFG1.5
5AI15.F58S1CASSGTGGQNTLYFG2.4
  • TCR cDNAs were amplified by PCR and were sequenced. Vβ gene families were assigned, and specific Vβ8 gene segments were numbered, according to Arden et al. (23). Jβ genes were assigned according to Malissen et al. (30) and Gascoigne et al. (31). FW, framework residues.