Table 2

Amino acid sequences of HBHA peptides

Amino terminusN-Ala-Glu-Asn-Ser-Asn-Ile-Asp-Asp-Ile-Lys-Ala-Pro-Leu-Leu-Ala-Ala-C 11
Peptide S1441N-Lys*-Ala-Glu-Gly-Tyr-Leu-Glu-Ala-Ala-Thr-CThis study
Peptide S1443N-Xxx-Glu-Gly-Tyr-Val-Asp-Gln-Ala-Val-Glu-Leu-Thr-Gln-Glu-Ala-Leu-Gly-CThis study
Peptide S1446N-Xxx-Gln-Glu-Xxx-Leu-Pro-Glu-Xxx-Leu-CThis study
Peptide S1447N-Phe-Thr-Ala-Glu-Glu-Leu-Arg-CThis study
  • * Lysine (4.6 pmol) and 11 pmol of alanine were found in the first sequencing cycle, probably because the tryptic peptide was partially cleaved at the Arg-Lys bond, giving rise to a mixture of two peptides, one starting with a lysine, one with an alanine. 

  • Xxx represents a position where no amino acid could be definitively assigned.