Table 1

Mean life span of a number of eat mutant strains

StrainGenotypenMeanDiff, %Significance level
Experiment no. 1
 N2+5021.6  ±  0.6
MQ581 eat-1(ad427) 5028.8  ±  1.0+33<0.0005
MQ582* eat-1(e2343) 5023.9  ±  0.9+110.03
 MQ573 eat-5(ad464) 5020.2  ±  0.5−60.07
MQ574 eat-7(ad450) 5014.0  ±  0.5−35<0.0005
Experiment no. 2
 N2+5019.5  ±  0.5
MQ643 eat-2(ad453) 5026.5  ±  0.7+36<0.0005
MQ594 eat-2(ad465) 5025.1  ±  0.7+29<0.0005
MQ644 eat-2(ad1113) 5028.4  ±  0.9+46<0.0005
MQ631 eat-2(ad1116) 3630.6  ±  1.4+57<0.0005
MQ646 eat-18(ad820sd) 5026.9  ±  1.0+38<0.0005
MQ649 eat-18(ad1110) 3822.4  ±  0.9+150.008
Experiment no. 3
 N2+5019.9  ±  0.5
MQ676 eat-3(ad426) 5022.0  ±  0.9+110.04
MQ584 eat-6(ad792) 5027.1  ±  0.6+36<0.0005
MQ583 eat-6(ad467) 5027.2  ±  0.7+37<0.0005
MQ591 eat-6(ad601) 5022.8  ±  0.7+150.001
MQ645 eat-6(ad997) 3923.6  ±  0.7+19<0.0005
 MQ677 eat-10(ad606) 3121.5  ±  0.8+80.12
MQ596 eat-13(ad522) 5026.0  ±  0.6+31<0.0005
  •  Sample size (n), mean life span ± SEM (in days), and percent difference from the wild type are given. The final column displays the probability that the mean life span is the same as the wild type (Student’s t test). Strains that lived significantly longer than the wild type (P < 0.05) are shown in bold, and short-lived strains are underlined. Three separate experiments were performed at different times, each with the wild type (N2) as a control. 

  • * A high proportion of eat-1(e2343) worms died from a prolapsed gonad. If these worms are subtracted from the analysis, the mean life span increases significantly. We also tested the life span of eat-8(ad599), eat-15(ad602), and eat-17(ad707); however, the sample sizes for these strains were too low to give reliable results because of high levels of matricidal hatching. eat-4(ad572), eat-4(ad819), and eat-4(ky5) also were tested, but most of the worms crawled off the plates and died from desiccation. The life span of DA531 eat-1(ad427), DA631 eat-3(ad426); him-8(e1489), DA464 eat-5(ad464), DA467 eat-6(ad467), and DA521 eat-7(ad450) were determined twice, and that of DA465 eat-2(ad465) was determined six times. The life span of these strains was very similar to respective backcrossed strains presented above.