Table 1

Phosphatase activity of recombinant SopB*

Substratenmol/min/mg protein
PtdIns 3-P47
PtdIns 3,4-P251
PtdIns 3,4,5-P368
Ins 1,3,4-P39.1
Ins 1,4,5-P313
Ins 1,3,4,5-P424
Ins 1,3,4,5,6-P54.5
  • * Assays were performed with 10 μM substrate in 50 mM Mops (pH 7.0), 10 mM EDTA, and 1 mM DTT.