Table 3

Tumorigenic activity of mutationally activated Met in NIH 3T3 cells under the control of the metallothionein promoter

MET construct*Tumorigenicity
No. mice with tumors/no. mice injectedMean tumor size in mm2
Wild type0/50
M1268T5/5234
Y1248H5/5238
  • * The wild-type construct encodes murine Met; M1268T and Y1248H encode mutationally activated murine Met. Each Met molecule is in vector 2999, which utilizes the metallothionein promoter. 

  • NIH 3T3 cells were cotransfected with the indicated construct plus pSV2 Neo, selected as pools of G418-resistant cells, and injected s.c. into nude mice at 5 × 105 cells per animal. Tumors were measured after 19 days. Mice injected with cells expressing wild-type Met developed tumors at 4–5 weeks.