Table 1

Transforming activity of Akt proteins

Akt constructTransformation of CEFTumorigenicity in chickensLatent period, weeks
None (vector control)0/4
c-Akt+1/74
v-Akt+++3/7≈1
Akt-Myr+++7/8≈1
Akt-E40K+++7/7≈1
Akt-K179MND
Akt-R25A+ND
Akt-T308D/S473D+0/4
Akt-T308A/S473AND
Akt-Myr-Δ11-60+++4/4≈1
Akt-Myr-R25A+++4/4≈1
Akt-Myr-T308A3/34
Akt-Myr-S473A++3/32-3
Akt-Myr-T308A/S473A0/3
Akt-Myr-K179M0/2
  • Akt proteins were expressed in the RCAS vector. This vector produces infectious avian retroviral progeny, and this progeny virus was used in the transformation tests. The degree of transformation was rated by the size of transformed cell foci at 14 days past infection and the amount of multilayering in such foci. ND, not determined. 

  • Tumors regressed.