Table 3

Effects of DnaJ and GrpE on the steady–state ATPase activity of DnaK channel mutants

Stimulation factors and kcat of ATP hydrolysis, s−1
Basal+DnaJ+DnaJ/GrpE
DnaK+1 (7.2 × 10−4)20240
YND145,147,148AAA1 (1.8 × 10−3)224
EV217,218AA1 (2.5 × 10−3)212
  • The stimulation factors are relative to the basal ATP hydrolysis rates, which are given in parentheses.