Table 4

Interdomain communication of DnaK channel mutants

koff of DnaK bound peptide, s−1Tryptophan fluorescence, λmax [nm]
−ATP+ATPADPATP
DnaK+1.17 × 10−32.31 (2,000-fold)344.6341.1
YND145,147,148AAA1.83 × 10−31.23 (700-fold)345.4342.6
EV217,218AA1.17 × 10−30.24 (200-fold)344.6342.5