Table 4

Complementation of icm mutants

Strain (Gene)Plasmid*Killing
JR32+++
25D
GS3008 (icmM)pMMB207αb
pGS-Lc-40 (icmM+L+K+)
pGS-Lc-47 (icmM+L+K+E+)++
pGS-Lc-47-D2 (icmL+K+E+)
pGS-Lc-47-D5 (icmM+L+K+)+
GS3009 (icmL)pMMB207αb
pGS-Lc-40 (icmM+L+K+)
pGS-Lc-47 (icmM+L+K+E+)++
pGS-Lc-47-D2 (icmL+K+E+)
pGS-Lc-47-D3 (icmM+K+E+)
pGS-Lc-47-D5 (icmM+L+K+)+
GS3010 (icmK)pMMB207αb
pGS-Lc-40 (icmM+L+K+)
pGS-Lc-47 (icmM+L+K+E+)++
pGS-Lc-47-D3 (icmM+K+E+)
pGS-Lc-47-D4 (icmM+L+E+)
pGS-Lc-47-D5 (icmM+L+K+)+
LELA4432 (icmE)pMMB207αb
pGS-Lc-42 (icmE+)+/−
pGS-Lc-47 (icmM+L+K+E+)++
pGS-Lc-47-D4 (icmM+L+E+)+
pGS-Lc-47-D5 (icmM+L+K+)+
  • * The plasmid pGS-Lc-47-D5 contains an in-frame deletion in icmE; the plasmid pGS-Lc-40 does not contain the icmE gene. 

  • As determined by macrophage cytotoxicity assay.