Table 3

Stabilities of the 8-fold TLP-ste mutant and a selection of enzymes from extremophiles

EnzymeSourceHalf-life, hrT, °CRef.
2-Ketoisovalerate-ferredoxin oxidoreductase Thermococcus litoralis 0.895(41)
Carbamoyl-phosphate synthetase Pyrococcus abyssi 395(42)
Sulfide dehydrogenase Pyrococcus furiosus 1295(43)
α-GlucosidaseThermococcus AN10.698(44)
3-Phosphoglycerate kinase Pyrococcus woesei 0.45100(45)
Glyceraldehyde-3-phosphate dehydrogenase Pyrococcus woesei 0.7100(46)
DNA-RNA polymerase Thermoproteus tenax 2100(26)
Hydrogenase Pyrococcus furiosus 2100(47)
Glyceraldehyde-3-phosphate dehydrogenase Thermotoga maritima >2100(48)
Eightfold TLP-ste mutant B. stearothermophilus 2.8100(This study)
ADP-dependent glucokinase Pyrococcus furiosus 3.6100(49)
Amylase Pyrococcus woesei 6100(26)
Glutamate dehydrogenase Pyrococcus furiosus 10100(50)
β-Glucosidase Pyrococcus furiosus 85100(51)
CellobiohydrolaseThermotoga sp.1108(26)
α-Amylase Pyrococcus furiosus 2120(52)