Table 1

Strains and plasmids

StrainRelevant genotypeSource or reference
E. coli
 RP437Wild type for motility(11)
 RP8611Δtsr, Δ(tar-tap), Δtrg(12)
 RP9352Δtsr, Δ(tar-tap), Δtrg, ΔcheZ(12)
 RP2867Δ(tap-cheB)(13)
 RP9535ΔcheA(J. S. Parkinson, Univ. of Utah, Salt Lake City)
 RP4782fliM182(14)
 RP4783fliM183(14)
 HCB429Δtsr, Δ(tar-tap), Δtrg(15)
S. typhimurium
 SJW1103Wild type for motility(16)
 KK2051cheATn10(17)
 MY107 fliG N72Y (18)
 SJW2323 fliG V135F (18)
Plasmids*
 pJC3-Tsr tsr + (J. S. Parkinson)
 pJC3-Tsr(R64C)tsr (190 C → T)This work
 pJC3-Tsr(T156P)tsr (466 A → C)This work
 pJC3-Tsr(A413V)tsr (1238 C → T)This work
 pNT201-Tar tar + (19)
 pNT201-Tar(R69K)tar (205-207 CGT → AAA)This work
 pNT201-Tar(T154I)tar (461 C → T)This work
 pMK76-Tar(M76K)tar (227 T → A)(20)
 pAL1-Trg trg + (21)
 pCG1-Tap tap + This work
 pPA56Cytoplasmic Tsr domain(ref. 22; see Methods)
 pMS103Cytoplasmic Tar domain(ref. 23; see Methods)
 pMS105Cytoplasmic Tar domain(ref. 23; see Methods)
  • * Genes on most plasmids are expressed from the tac promoter; pMS plasmids use the trc promoter, whereas pMK76 uses the natural Tar promoter. The numbers in parentheses under “relevant genotype” of plasmids refer to the nucleotide positions, starting at the first nucleotide of the ATG codon.