Table 1

Reduced occluded virus production in SF-21 cells expressing human PKR and infected with a baculovirus mutant lacking pk2

Plasmid 1Plasmid 2VirusCells with occluded virus, %
L197.0 ± 1.0
vKINdel98.0 ± 0.5
PKRL139.9 ± 0.3
PKRpk2L142.3 ± 0.7
PKRvKINdel15.6 ± 1.2
PKRpk2vKINdel37.3 ± 1.1
  • SF-21 cells were serially transfected with plasmids 1 and 2 expressing PKR and pk2, respectively, and then infected with either wild-type AcMNPV (L1 strain) or pk2-deleted (vKINdel) virus. At 48 h after infection, photographs were taken of two or three fields on the plate. For each field, the total number of cells and the number of cells containing occluded virus were counted. Results are expressed as the percentage of cells expressing occluded virus (mean ± SEM).