Table 2

Percent of ASH neuron dye-filling defects in 3- and 8-day-old transgenic animals

Constructs (no. of lines)3-day-old animals8-day-old animals
OSM-10∷GFP (5)0%  (163) 1 ± 1%  (225)
OSM-10∷GFP/Htn-Q2 (5)0%  (168)0%  (203)
OSM-10∷GFP/Htn-Q23 (6)0%  (204)0%  (185)
OSM-10∷GFP/Htn-Q95 (4)0%  (169) 5 ± 3%  (189)
OSM-10∷GFP/Htn-Q150 (5)3 ± 1%  (155)27 ± 6%  (235)
  • Dye-filling experiments, using the specified transgenic animals were performed as described in Materials and Methods. The percentage of ASH neurons defective for DiD staining and positive for OSM-10∷GFP staining is reported. The number of ASH neurons scored is in parentheses. SEM also is reported.