Table 1

Ratio of normal:arrested seen in FIS/fis2 plants segregating for the cosmid 18H1

LineKanR progeny (+18H1) KanS progeny (−18H1)
 1179:570.051Not tested
 6153:550.23Not tested
10210:730.1 54:61 0.43
Control C24NA211:2160.058
  • KanR plants contain the cosmic and KanS do not. Each line is derived from an independent transformation event. Expected ratios for KanR and KanS plants are 75:25 and 50:50, respectively, if the 18H1 cosmid complements the fis2 mutation. Control cross with C24 shows the segregation of fis2 without complementation. NA, not available.