Table 2

The yields of parent, D199A mutant, and rescued viruses from quiescent and serum-starved human lung fibroblasts harvested at 5 or 20 hr after infection

HLF cellsHr after infectionYield*
HSV-1(F)R7914R7915
Serum starved51.2  ×  1042.4  ×  1041.3  ×  104
206.4  ×  1074.0  ×  1065.6  ×  107
Quiescent56.1  ×  1045.5  ×  1044.1  ×  104
201.8  ×  1081.1  ×  1071.7  ×  108
  • * Diploid human lung fibroblasts (HLF) grown in 25-cm2 flasks were either serum-starved in medium containing 0.25% FCS for 5 days or allowed to become fully contact inhibited (quiescent) and maintained in same medium for 7 days. The cells were infected (0.05 PFU/cell) in the same medium in which they were maintained before infection.