Table 2

RocG-GlutDH activity

Strain (genotype)Carbon and nitrogen sourcesRocG activity
BB1268 (wild-type)Succinate + glutamate<40
BB1268 (wild-type)Succinate + glutamate + ornithine649
BB1552 (sigL::aphA3)Succinate + glutamate + ornithine<40
BB1553 (rocRΔ3::aphA3)Succinate + glutamate + ornithine<40
BB1542 [rocR(T120I)]Succinate + glutamate763
BB1542 [rocR(T120I)]Succinate + glutamate + ornithine829
BB1680 (ahrC::aphA3)Succinate + glutamate + ornithine<40
  • The null mutations in sigL, rocR, rocD, and ahrC and the constitutive rocR(T120I) mutation were as described (10, 13, 14). All strains carried the ΔgudB::tet mutation to avoid any contribution from GudB-GlutDH(17).