Table 4

Expression of a rocG-lacZ fusion containing the DAS/UAS in the upstream position

Strain (genotype)β-galactosidase activity in different media
Succinate GlutamateSuccinate Glutamate Ornithine
BB1501 (wild-type)2.1264.9
BB1503 (sigL::aphA3)1.21.2
BB1502 (rocRΔ3::aphA3)2.01.4
BB1522 [rocR(T120I)]122.6225.3
  • Strain BB1501 and its derivatives carry a rocG-lacZ fusion [as in pBB986, (Fig. 5)] at the amyE locus.