Table 1

Thermal stabilities (Tm) of PNA·DNA and PNA·PNA complexes

PNA sequence (PNA number)Tm, °C
Vs. DNA*Vs. PNA
H-GTAGATCACT-Lys-NH25168
H-GTAGDTCACT-Lys-NH25771 (64)
H-GTDGDTCDCT-Lys-NH26781 (49)§
H-Lys-GsUDGDsUCDCsU-Lys-NH2 (1495)5874
H-AGTGATCTAC-Lys-NH24968
H-AGTGAsUCTAC-Lys-NH24668
H-AGUsGAsUCsUAC-Lys-NH24468
H-Lys-DGsUGDsUCsUDC-Lys-NH2 (1496)5874
H-Lys-GsUDGDsUCDCT-Lys-NH2 (1674)65.5
H-Lys-AGsUGDsUCsUDC-Lys-NH2 (1672)56
H-Lys-GsUAGDsUCDCT-Lys-NH2 (1675)61
H-Lys-AGsUGDsUCTDC-Lys-NH2 (1673)58
H-Lys-GsUDGGsUCDCsU-Lys-NH2 (1837)67
H-Lys-DGUsGDCCsUDC-Lys-NH2 (1838)66
H-Lys-DCGDCsUCDCsU-Lys-NH2 (1657)67
H-Lys-DGsUGDGsUCGsU-Lys-NH2 (1658)68
  • * The complementary DNA oligonucleotides 5′-d(AGTGATCTAC) and 5′-d(GTAGATCACT) were used. Thermal stabilities (Tm) were measured in 10 mM sodium phosphate/100 mM NaCl/0.1 mM EDTA, pH 7.0, at a heating rate of 0.5 °C per step (0.7°C/min) (5–90°C). 

  • The complementary PNA oligomers H-AGTGATCTAC-Lys-NH2 and H-GTAGATCACT-Lys-NH2 were used. 

  • Measured vs. H-AGTGAsUCTAC-Lys-NH2

  • § Measured vs. H-AGsUGAsUCsUAC-Lys-NH2