Table 1

cAMP levels in HAEC treated with oxidized phospholipids

ConditioncAMP
Control5.1  ±  0.6
POVPC8.6  ±  0.4*
PGPC5.0  ±  0.4
IsoP15.1  ±  1.5*
  • HAEC were preincubated for 10 min with 5 μmol/liter isobutyl-1-methylxanthine then POVPC (5 μg/ml), or isoproterenol (5 μmol/liter) were added for 1 h. Levels of cAMP were determined by using RIA. Values represent mean ± SD of quadruplicate samples. Values are given as pmol/mg protein per min. These data are representative of three experiments. *, P < 0.05. IsoP, isoproterenol.