Table 1

Plasmids and strains employed in this study

Plasmid nameRelevant featuresSource
pAID135PhoA(Δ2-22) under control of tac promoterRef. 18
pΔA-uPaΔA-uPA under control of tac promoterRef. 14
pFA2GrxA in pBAD33 under its own RBSThis work
pFA3TrxA in pBAD33 under its own RBSRef. 19
pFA4TrxA(P34S) in pBAD33This work
pFA5TrxA(G33P,P34Y) in pBAD33Ref. 19
pfA6TrxA(G33P,P34H) in pBAD33Ref. 19
pFA7TrxA(P34H) in pBAD33Ref. 19
pFA8TrxA(C35A) in pBAD33Ref. 19
pFA21TrxB in pBAD33 under its own RBSThis work
pFA22trxB upstream region in pFA21 at NsiI siteThis work
pFA28trxB upstream region, araC, ParaB-trxB in pKOVThis work
pBADdsbADsbA in pBAD33 with optimized RBSThis work
pBADdsbCDsbC in pBAD33 with optimized RBSThis work
pBADΔSSdsbADsbA(Δ2-19) in pBAD33 with optimized RBSThis work
pBADΔSSdsbCDsbC(Δ2-20) in pBAD33 with optimized RBSThis work
pBADrPDImature rat PDI in pBAD33 with optimized RBSThis work
pTrcStIItPAhuman tPA with StII leader in pTrc99AJi Qiu, University of Texas
pTrcStIIvtPAtPA(Δ6-175) with StII leader in pTrc99AThis work
pTrctPAtPA in pTrc99AThis work
pTrcvtPAtPA(Δ6-175) in pTrc99AThis work
pBAD33araBAD promoter, CmR, pACYC oriRef. 16
pTrc99Atrc promoter, AmpR, ColE1 oriAmersham Pharmacia
pKOVcounter-selectable allele exchange vectorG. Church laboratory, Harvard University
StrainGenotypeSource
DHB4MC1000 ΔphoA(PvuII) phoR ΔmalF3 F′[lac+(lacIQ) pro]Ref. 15
WP597DHB4 trxB::KmW. Prinz, Harvard University
FA112DHB4 gshA20::Tn10Km trxB::Km… Tn10 suppThis work
FA113DHB4 gor522…mini-Tn10Tc trxB::Km suppThis work
FA196DHB4 ΔtrxB::ParaB-trxBThis work
FA222FA196 gor522…mini-Tn10TcThis work