Table 2

Non-Mendelian segregation

GenotypeAllele
his4-X/HIS4MATa/MATα
5:36:22:63:5% NMS% PMS/total5:36:22:63:5% NMS% PMS/total
Wild type06501.1 (11/1,018)
00400.4 (4/998)
pms1 57312.5 (16/638) 37.5 (6/16) 27422.3 (15/637) 26.7 (4/15)
mlh1 10127124.3 (42/978)*
52.3 (22/42)57912.2 (22/981) 27.3 (6/22)
mlh2 05100.9 (6/696)
06401.4 (10/696)
mlh3 07701.3 (14/1,093)
01400.5 (5/1,094)
  • Non-Mendelian segregation was analyzed for tetrads with four viable spores. Patterns of segregation were assigned according to the nomenclature used for eight spored fungi, 5:3 and 3:5 segregations have a single-sectored colony, indicating unrepaired heteroduplex. For MAT segregations, 6:2 and 2:6 segregations were arbitrarily assigned to the aaaα and αααa patterns, respectively. The his4-X mutation will create a 4-bp heterology in hybrid DNA; MATa/MATα theoretically creates a 642/747-bp double heterology. 

  • * One 0:8 segregation was observed.