Table 1

Complementation of E. coli lysS lysU double mutants by genes encoding class I LysRS proteins

PlasmidGrowth conditions*
30°C40°C40°C + Lys42°C42°C + Lys
pCBS1-Bs trpS++
pKS-Ec lysS++++±±
pCBS1-Mm lysS++±
pCBS1-Bb lysS++++
  • ++, growth after 24 h; +, growth after 48 h; ±, growth after 72 h; −, no growth. 

  • * Growth was on Luria–Bertani agar (100 μg/ml ampicillin) with the addition of 5 mM l-lysine where indicated.