Table 2

Recognition of tRNA by M. maripaludis LysRS

tRNAKM tRNA, μMKM Lys, μMkcat, S−1
M. maripaludis * 0.11 ± 0.032.2 ± 0.20.2 ± 0.05
M. maripaludis-CNBr0.08 ± 0.0125.0 ± 8.00.05 ± 0.01
E. coli 3 ± 0.8350 ± 470.004 ± 0.0004
E. coli-CNBr0.25 ± 0.05230 ± 480.002 ± 0.0001
  • * M. maripaludis total tRNA (40 pmol tRNALys/A260). 

  • tRNA was treated with CNBr as described. 

  • E. coli tRNALys