Table 3

Recognition of tRNALys by B. burgdorferi LysRS

tRNALysB. burgdorferi LysRS Vmax/KM, relative*E. coli LysRS Vmax/KM, relative
Wild-type11
Anticodon
 UCU<0.04<0.01
 UAC<0.04<0.01
 UUA<0.04<0.01
 UUG0.080.22
 GUU0.340.059
Discriminator
 G730.280.11
 C730.280.10
 U730.290.12
  • * For the wild-type tRNALys, kcat = 0.22 ± 0.03 min−1 and KM = 1.5 ± 0.6 μM. 

  • Values taken from ref. 33 (for wild-type, kcat = 3.32 min−1 and KM = 1.9 μM).