Table 4

Recognition of in vitro transcribed tRNALys variants by B. burgdorferi and E. coli LysRS

LysRStRNAKM tRNA, μMkcat, min−1kcat/KM, R*
B. burgdorferi B. burgdorferi 1.98 ± 0.3247.4 ± 4.21
E. coli wild type1.5 ± 0.60.22 ± 0.003160
E. coli G2⋅U713.9 ± 0.60.21 ± 0.02432
E. coli B. burgdorferi 4.3 ± 0.40.21 ± 0.01490
E. coli wild type1.93.3214
E. coli G2⋅U716.3 ± 0.80.15 ± 0.0081022
  • * Relative value expressed as fold decrease in kcat/KM compared to aminoacylation of B. burgdorferi tRNA1Lys transcript by B. burgdorferi LysRS. 

  • From ref. 33