Table 1

Methyllysine/lysine ratio in H3

H3Site of methylation
4 % Methylated9 % Methylated27 % Methylated
Tetrahymena 47None detectedNone detected
Yeast34*None detectedNot determined
HeLa733Not determined
  • Levels of unmodified and mono- or dimethyllysine were determined by N-terminal sequencing followed by RP-HPLC analysis. Peaks representing either lysine or methyllysine were integrated and converted to % methyllysine at the residue indicated. Unless otherwise stated, values represent monomethyllysine. 

  • *Both mono- and dimethyllysine were detected (19% and 15%, respectively).