Table 1

Detection of carbonic anhydrases in microbes

SpeciesMolecular mass in Western blotting,a kDa Specific activity,b units/mg
α-classβ-classγ-class
Bacteria domainc
Acetobacterium woodiid22197.3  ±  0.6
Bacillus subtilisd * * <0.01
Carboxydothermus hydrogenoformansdk2319<0.01
Clostridium thermoaceticumdk24181.0  ±  0.1
Helicobacter pylorie23l25l<0.01
Methylobacterium extorquensf23191.4  ±  0.2
Pseudomonas aeruginosag23l, 25l*20l*<0.01
Rhizobium melilotif24181.5  ±  0.1
Rhodobacter capsulatusf23l18l1.5  ±  0.2
Rhodobacter sphaeroidesf23181.6  ±  0.2
Rhodospirillum rubrumf21193.6  ±  0.2
Salmonella typhimuriumg2226l * 0.6  ±  0.2
Staphylococcus aureusd231.2  ±  0.1
Thermotoga maritimahk0.01
Vibrio fischerig191.5  ±  0.3
Archaea domainc
Methanobacterium formicicumi21<0.01
Methanobacterium thermoautotrophicum ΔHik21l17l0.8  ±  0.2
Methanobacterium thermoautotrophicum Marburgik21171.3  ±  0.1
Methanococcus jannaschiijk * 0.3  ±  0.1
Methanococcus maripaludisi<0.01
Methanosaeta conciliii213.0  ±  0.4
Methanosarcina barkerii371.4  ±  0.2
Methanosarcina thermophilajk37l1.8  ±  0.2
Methanospirillum hungateiii247.1  ±  0.5
Natronococcus occultusi0.01
Pyrococcus furiosusik20l<0.01
Sulfolobus solfataricusjk23191.6  ±  0.2
  • a Approximate molecular masses of cross-reactive proteins. A minus sign (−) indicates no crossreactive proteins were detected. An asterisk (*) indicates that crossreactive protein expected from sequence data was not detected. 

  • b Specific activity was measured at 23°C for mesophiles and 55°C for thermophiles. One unit = (t0t)/t, where t0 (uncatalyzed reaction) and t (catalyzed reaction) are the times required for the pH to decrease from 7.8 to 7.0. 

  • c Species from the Bacteria domain belong to the following kingdoms: Gram-positive,d proteobacteria (ɛ class),e proteobacteria (α class),f proteobacteria (γ class),g or thermotoga.h Species from the Archaea domain belong to the following kingdoms: Euryarchaeotai and Crenarchaeotaj

  • k Thermophilic species. 

  • l The molecular mass detected by Western blotting is similar to the molecular mass calculated from the deduced amino acid sequence.