Table 1

Summary of mutant screens using TEV-bar

ScreenMutagen*Number screenedGA survivorsCapsid positiveCol-0 ecotype§M3 progeny susceptible
1EMS7,4007433
2FNB16,90012100ND
3EMS13,6003111
4EMS12,0005110
5EMS13,2507666
6EMS13,00019311
7EMS5,000113**3ND
Total81,15064281511
  • * EMS, ethyl methanesulfonate; FNB, fast-neutron bombarded. 

  • Number of plants that regenerated inflorescence tissue after GA application. 

  • Number of GA survivors that possessed capsid protein in newly regenerated inflorescence tissue. 

  • § Number of TEV capsid protein-positive GA survivors that were verified to be of the Col-0 ecotype based on the genotype for four different SSLP markers (nga6, nga76, nga111, and nga172). 

  • Number of capsid protein-positive Col-0 M2 GA survivors whose M3 progeny were systemically infected by TEV-GUS. These plants were considered to have heritable mutations resulting in a gain-of-susceptibility phenotype after TEV infection. 

  • ND, not determined. 

  • ** In screen 7, only the three GA survivors that had the Col-0 genotype were tested for TEV capsid protein in systemic tissue. Therefore, a total of 56 GA survivors were tested for systemic movement by TEV.