Table 1

FBP levels and the quarternary structure of M2-PK in E7-expressing NIH3T3 cells

Cell typeFBP, nmole/ mg proteinTetramer, %Dimer, %
M/10.22  ± 0.0463.5  ± 0.336.5  ± 0.6
Δ79-830.40  ± 0.10n.s.59.9  ± 1.6n.s.40.1  ± 1.6n.s.
E7/20.40  ± 0.0447.2  ± 0.952.8  ± 0.8
 (P < 0.05) (P < 0.001) (P < 0.001)
  • Control and E7-expressing NIH3T3 subclones were used for gel filtration analysis of M2-PK as indicated; the results are given as the relative amounts of the tetrameric and dimeric forms of M2-PK (Embedded Image ± SEM, n = 4). FBP levels were determined in perchloric acid extracts. Embedded Image ± SEM, n = 5. n.s., not significant.