Table 2

E7-dependent alterations of intracellular concentrations of glycolytic metabolites in 14/2 cells

MetabolitesEmbedded Image ± SEM
E7 offE7 onSignificance
FBP, nmole/mg protein13.4  ± 0.628.3  ± 5.9P < 0.5
PEP, nmole/mg protein0.9  ± 0.11.7  ± 0.2P < 0.05
IMP, nmole/mg protein0.2  ± 0.42.0  ± 1.7P < 0.05
[ATP] : [ADP]5.2  ± 1.43.5  ± 0.6P < 0.05
Embedded Image4.1  ± 0.91.9  ± 0.2P < 0.001
Embedded Image19.36.8
  • The concentration of various metabolites is given for mock-treated and dexamethasone-treated 14/2 cells. In each case, the significance of dependence on E7 expression was calculated as described (8, 12). n = 5. The term (pyruvate⋅ATP):(PEP⋅ADP) was calculated from the concentrations of the individual metabolites.