Table 1

Polyketides produced by AT and KR substitutions in DEBS modules 2, 5, and 6

Mutation6dEB analog productCompound no.Yield*
Module 2
rapAT210-desmethyl 2 0.2
 AT/ACP linkerNP
rapDH/KR410,11-anhydro 3 0.02
rapDH/ER/KR111-deoxy 4 0.2
Module 5
rapAT24-desmethyl 7 0.04
 AT/ACP linker5-deoxy-5-oxo 8 0.1
rapDH/KR44,5-anhydro 9 ND
rapDH/ER/KR15-deoxy-5-oxo; 5-deoxy 8, 10 0.5, 0.04
Module 6
rapAT22-desmethyl 11 0.7
 AT/ACP linker3-deoxy-3-oxo; 2-desmethyl-3-deoxy-3-oxo 12, 14 0.3, 0.4
rapDH/KR42,3-anhydro 13 0.4
rapDH/ER/KR13-deoxy-3-oxo; 2,3-anhydro 12, 13 0.3, 0.2
Module 2 (AT+KR)
rapAT2+AT/ACP linkerNP
rapAT2+rapDH/KR410-desmethyl-10,11-anhydro 5 <0.005
rapAT2+rapDH/ER/KR110-desmethyl-11-deoxy 6 <0.005
Module 6 (AT+KR)
rapAT2+AT/ACP linker2-desmethyl-3-deoxy-3-oxo 14 0.2
rapAT2+rapDH/KR42-desmethyl-3-(epi) 15 ND
rapAT2+rapDH/ER/KR12-desmethyl-3-deoxy-3-oxo 14 ND
  • * Relative to 6dEB (1) under similar conditions (≈20 mg/liter). ND, not determined; NP, no product.